Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA
Keywords
Compared with sham controls, exposed rats showed brain vascular alterations and significant edema (p<0.05). DNA damage in brain tissue was higher in the exposed group (18.26% tail DNA) than controls (4.06% tail DNA). Testicular tissue showed significant chang…
Study snapshot
Abstract
Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA damage of brain and qRT-PCR analysis Yavas MC, Kilitci A, Çelik E, Yegin K, Sirav B, Varol S. Rat brain and testicular tissue effects of radiofrequency radiation exposure: Histopathological, DNA damage of brain and qRT-PCR analysis. INTERNATIONAL JOURNAL OF RADIATION RESEARCH. JUL 2024. 22(3):529-536. doi: 10.61186/ijrr.22.3.529 Abstract We evaluate the effects of radiofrequency electromagnetic field (RF-EMF) on rat brain and testicular tissue using histopathology, comet assay, and real-time quantitative PCR techniques. Materials and Methods: Two equal groups of fourteen rats one for sham-control and the other for exposure (n = seven) were created. For a duration of 14 days, the exposure group (2100 MHz, testicular tissue SAR values of 163 mW/kg for 10 g, brain tissue SAR values of 292 mW/ kg on average) was subjected to five hours of exposure per day. Evaluations were conducted on tissue gene expression levels, histopathology, and DNA damage to brain tissue. Results: The histological examination of brain tissue from the exposed group revealed vascular alterations and significant edema (p < 0.05). It was determined that RF radiation-induced much more cellular damage in the exposed group (18.26% tail DNA) than in the control group (4.06% tail DNA). Signs of deterioration in spermatogenic cells in the testicular tissue of the exposed group also changed significantly (p < 0.05). The Bax and bcl-2 genes showed a significant difference (p < 0.05) in the mRNA level data, whereas the p53 genes showed no significant change (p > 0.05). Conclusion: These findings suggest that it may cause some histopathological and cellular damage in brain and testis tissue. Open access paper: ijrr.com
AI evidence extraction
Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.
Main findings
Compared with sham controls, exposed rats showed brain vascular alterations and significant edema (p<0.05). DNA damage in brain tissue was higher in the exposed group (18.26% tail DNA) than controls (4.06% tail DNA). Testicular tissue showed significant changes consistent with deterioration in spermatogenic cells (p<0.05), and Bax and bcl-2 mRNA differed significantly while p53 showed no significant change.
Outcomes measured
- Brain histopathology (vascular alterations, edema)
- Brain DNA damage (comet assay; % tail DNA)
- Testicular histopathology (spermatogenic cell deterioration)
- Gene expression (qRT-PCR; Bax, bcl-2, p53 mRNA)
Limitations
- Small group sizes (n=7 per group)
- Exposure source/setup not described in the abstract
- Outcomes for DNA damage reported for brain tissue; testicular DNA damage not reported in the abstract
View raw extracted JSON
{
"study_type": "animal",
"exposure": {
"band": "RF",
"source": null,
"frequency_mhz": 2100,
"sar_wkg": 0.292,
"duration": "5 hours/day for 14 days"
},
"population": "Rats",
"sample_size": 14,
"outcomes": [
"Brain histopathology (vascular alterations, edema)",
"Brain DNA damage (comet assay; % tail DNA)",
"Testicular histopathology (spermatogenic cell deterioration)",
"Gene expression (qRT-PCR; Bax, bcl-2, p53 mRNA)"
],
"main_findings": "Compared with sham controls, exposed rats showed brain vascular alterations and significant edema (p<0.05). DNA damage in brain tissue was higher in the exposed group (18.26% tail DNA) than controls (4.06% tail DNA). Testicular tissue showed significant changes consistent with deterioration in spermatogenic cells (p<0.05), and Bax and bcl-2 mRNA differed significantly while p53 showed no significant change.",
"effect_direction": "harm",
"limitations": [
"Small group sizes (n=7 per group)",
"Exposure source/setup not described in the abstract",
"Outcomes for DNA damage reported for brain tissue; testicular DNA damage not reported in the abstract"
],
"evidence_strength": "low",
"confidence": 0.78,
"peer_reviewed_likely": "unknown",
"keywords": [
"radiofrequency electromagnetic field",
"RF-EMF",
"2100 MHz",
"SAR",
"rat",
"brain",
"testis",
"histopathology",
"comet assay",
"DNA damage",
"qRT-PCR",
"Bax",
"bcl-2",
"p53"
],
"suggested_hubs": [
{
"slug": "animal-studies",
"weight": 0.9,
"reason": "Experimental RF-EMF exposure study in rats assessing tissue effects."
}
]
}AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.
Identifiers and source
DOI: 10.61186/ijrr.22.3.529
PubMed: Not supplied