The endocannabinoid system is involved in the anxiety-like behavior induced by dual-frequency
Keywords
Single-frequency EMR at 2.65 GHz or 0.8 GHz did not induce anxiety-like behavior in mice, whereas dual-frequency EMR at 2.65/0.8 GHz significantly induced anxiety-like behavior. Dual-frequency exposure was associated with increased serum corticosterone and CR…
Study snapshot
Abstract
The endocannabinoid system is involved in the anxiety-like behavior induced by dual-frequency 2.65/0.8 GHz electromagnetic radiation in mice Xue T, Ma RH, Xu C, Sun B, Yan DF, Liu XM, Gao D, Li ZH, Gao Y, Wang CZ. The endocannabinoid system is involved in the anxiety-like behavior induced by dual-frequency 2.65/0.8 GHz electromagnetic radiation in mice. Front Mol Neurosci. 2024 Apr 15;17:1366855. doi: 10.3389/fnmol.2024.1366855. Abstract As wireless communication devices gain popularity, concerns about the potential risks of environmental exposure to complex frequency electromagnetic radiation (EMR) on mental health have become a public health issue. Historically, EMR research has predominantly focused on single- frequency electromagnetic waves, neglecting the study of multi-frequency electromagnetic waves, which more accurately represent everyday life. To address these concerns, our study compared the emotional effects of single- frequency and dual-frequency EMR while exploring potential molecular mechanisms and intervention targets. Our results revealed that single-frequency EMR at 2.65 or 0.8 GHz did not induce anxiety-like behavior in mice. However, exposure to dual-frequency EMR at 2.65/0.8 GHz significantly led to anxiety- like behavior in mice. Further analysis of mouse sera revealed substantial increases in corticosterone and corticotrophin releasing hormone levels following exposure to 2.65/0.8 GHz EMR. Transcriptome sequencing indicated a significant decrease in the expression of Cnr1, encoding cannabinoid receptor 1 Type (CB1R), in the cerebral. This finding was consistently verified through western blot analysis, revealing a substantial reduction in CB1R content. Additionally, a significant decrease in the endocannabinoid 2-arachidonoylglycerol was observed in the cerebral cortex. Remarkably, administering the cannabinoid receptor agonist Win55-212-2 significantly alleviated the anxiety-like behavior, and the cannabinoid receptor antagonist AM251 effectively counteracted the anti-anxiety effects of Win55-212-2. In summary, our research confirmed that dual-frequency EMR is more likely to induce anxiety-like behavior in mice than single-frequency EMR, with implications for the hypothalamic– pituitary–adrenal axis and the endocannabinoid system. Furthermore, our findings suggest that Win55- 212-2 may represent a novel avenue for researching and developing anti-EMR drugs. Open access paper: frontiersin.org
AI evidence extraction
Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.
Main findings
Single-frequency EMR at 2.65 GHz or 0.8 GHz did not induce anxiety-like behavior in mice, whereas dual-frequency EMR at 2.65/0.8 GHz significantly induced anxiety-like behavior. Dual-frequency exposure was associated with increased serum corticosterone and CRH, decreased Cnr1/CB1R expression and CB1R protein, and decreased 2-AG in cerebral cortex; the CB receptor agonist Win55-212-2 alleviated anxiety-like behavior and AM251 counteracted this effect.
Outcomes measured
- Anxiety-like behavior
- Serum corticosterone
- Serum corticotrophin releasing hormone (CRH)
- Cnr1 (CB1R) gene expression (transcriptome)
- CB1R protein content (western blot)
- 2-arachidonoylglycerol (2-AG) levels in cerebral cortex
- Pharmacologic modulation effects (Win55-212-2 agonist; AM251 antagonist)
Limitations
- Sample size not reported in provided abstract/metadata
- Exposure metrics (e.g., SAR, power density) and duration not reported in provided abstract/metadata
- Animal model; generalizability to humans not established in provided abstract/metadata
- Behavioral assay details and blinding/randomization not described in provided abstract/metadata
View raw extracted JSON
{
"study_type": "animal",
"exposure": {
"band": "RF",
"source": "wireless communication devices (environmental exposure model)",
"frequency_mhz": null,
"sar_wkg": null,
"duration": null
},
"population": "Mice",
"sample_size": null,
"outcomes": [
"Anxiety-like behavior",
"Serum corticosterone",
"Serum corticotrophin releasing hormone (CRH)",
"Cnr1 (CB1R) gene expression (transcriptome)",
"CB1R protein content (western blot)",
"2-arachidonoylglycerol (2-AG) levels in cerebral cortex",
"Pharmacologic modulation effects (Win55-212-2 agonist; AM251 antagonist)"
],
"main_findings": "Single-frequency EMR at 2.65 GHz or 0.8 GHz did not induce anxiety-like behavior in mice, whereas dual-frequency EMR at 2.65/0.8 GHz significantly induced anxiety-like behavior. Dual-frequency exposure was associated with increased serum corticosterone and CRH, decreased Cnr1/CB1R expression and CB1R protein, and decreased 2-AG in cerebral cortex; the CB receptor agonist Win55-212-2 alleviated anxiety-like behavior and AM251 counteracted this effect.",
"effect_direction": "harm",
"limitations": [
"Sample size not reported in provided abstract/metadata",
"Exposure metrics (e.g., SAR, power density) and duration not reported in provided abstract/metadata",
"Animal model; generalizability to humans not established in provided abstract/metadata",
"Behavioral assay details and blinding/randomization not described in provided abstract/metadata"
],
"evidence_strength": "low",
"confidence": 0.74,
"peer_reviewed_likely": "yes",
"keywords": [
"dual-frequency",
"2.65 GHz",
"0.8 GHz",
"RF-EMR",
"anxiety-like behavior",
"mice",
"HPA axis",
"corticosterone",
"CRH",
"endocannabinoid system",
"CB1R",
"Cnr1",
"2-AG",
"Win55-212-2",
"AM251"
],
"suggested_hubs": [
{
"slug": "animal-studies",
"weight": 0.9,
"reason": "Mouse study assessing behavioral and molecular effects of RF electromagnetic radiation."
}
]
}AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.
Identifiers and source
DOI: 10.3389/fnmol.2024.1366855
PubMed: Not supplied